FAQ
Posted on April 1, 2020
Are there any calcium indicators that don't require probenecid (PBC)?
Calcium dyescalcium fluxcalcium indicatorfluorescent calcium indicatorno probenecid
Are there any calcium indicators that don't require probenecid (PBC)?
Calcium dyescalcium fluxcalcium indicatorfluorescent calcium indicatorno probenecid
Posted on February 19, 2020
Why is my Fluo-4 signal not at a useful magnitude?
Calcium dyescalcium indicatorDissociation Constant (Kd)cell-permeable probesfluorescence imaging
Why is my Fluo-4 signal not at a useful magnitude?
Calcium dyescalcium indicatorDissociation Constant (Kd)cell-permeable probesfluorescence imaging
Posted on February 19, 2020
Why are my cells leaking Fluo-4 AM?
AM esterCalcium dyescalcium indicatorcell-permeable probesdegree of labelingfluo-4
Why are my cells leaking Fluo-4 AM?
AM esterCalcium dyescalcium indicatorcell-permeable probesdegree of labelingfluo-4
Posted on February 19, 2020
What are possible reasons for inconsistent Fluo-4 AM loading?
AM esterCalcium dyesGreen Fluorescencecalciumcalcium indicatorcell linescell-permeable probescell culturedegree of labelingfluo-4
What are possible reasons for inconsistent Fluo-4 AM loading?
AM esterCalcium dyesGreen Fluorescencecalciumcalcium indicatorcell linescell-permeable probescell culturedegree of labelingfluo-4
Posted on February 19, 2020
How do I minimize cell damage during Fluo-4 AM loading?
AM esterCalcium dyesCell ViabilityGreen Fluorescencecalcium indicatorcell damagecell-permeable probesfluo-4
How do I minimize cell damage during Fluo-4 AM loading?
AM esterCalcium dyesCell ViabilityGreen Fluorescencecalcium indicatorcell damagecell-permeable probesfluo-4
Posted on December 10, 2019
Why is probenecid used to conduct calcium flux assays when using fluorescent calcium indicators in certain cell types?
Ca2+calciumcalbrytecell assayprobenecidcalbryte 520no probenecidcalcium mobilitycalcium indicatororganic anion transporter
Why is probenecid used to conduct calcium flux assays when using fluorescent calcium indicators in certain cell types?
Ca2+calciumcalbrytecell assayprobenecidcalbryte 520no probenecidcalcium mobilitycalcium indicatororganic anion transporter
Posted on June 26, 2019
What is the working mechanism of Ionomycin?
calciumcalcium indicatorGPCRcalcium fluxcalcium mobilityIonomycinionophore
What is the working mechanism of Ionomycin?
calciumcalcium indicatorGPCRcalcium fluxcalcium mobilityIonomycinionophore
Posted on June 26, 2019
Why do CHO cells require the use of probenecid in calcium assays?
calcium fluxcalcium indicatorcalciumcell assayprobenecidorganic anion transporterCHOGPCR
Why do CHO cells require the use of probenecid in calcium assays?
calcium fluxcalcium indicatorcalciumcell assayprobenecidorganic anion transporterCHOGPCR
Posted on June 13, 2019
When performing calcium flux assay in living cells with fluorescent calcium indicators, can you use a fluorescent plate reader that does not have a kinetic reading function or a built-in liquid handler?
calciumCa2+calcium indicatorcalcium fluxcalcium mobilitycell assayfluorescent plate readerendpointno washno probenecidGPCR
When performing calcium flux assay in living cells with fluorescent calcium indicators, can you use a fluorescent plate reader that does not have a kinetic reading function or a built-in liquid handler?
calciumCa2+calcium indicatorcalcium fluxcalcium mobilitycell assayfluorescent plate readerendpointno washno probenecidGPCR
Posted on June 13, 2019
What's the kd of Calcium Indicators including Fluo-8, Cal-520 and Calbryte indicators?
calcium indicatorcalbryte 520
What's the kd of Calcium Indicators including Fluo-8, Cal-520 and Calbryte indicators?
calcium indicatorcalbryte 520
Posted on June 13, 2019
What is the pH value of your probenecid solution (Cat# 20062)?
calciumprobenecidcalbryte 520calcium indicator
What is the pH value of your probenecid solution (Cat# 20062)?
calciumprobenecidcalbryte 520calcium indicator
Posted on June 13, 2019
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
Calcium dyescalcium concentrationcalcium indicatorfluorescence imagingfluorescent calcium indicatorcalbryte 520
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
Calcium dyescalcium concentrationcalcium indicatorfluorescence imagingfluorescent calcium indicatorcalbryte 520
Posted on June 12, 2019
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
calcium fluxcalbryte 520calcium indicatormitochondria membrane potential
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
calcium fluxcalbryte 520calcium indicatormitochondria membrane potential
Posted on June 12, 2019
Can I fix the cells before or after addition of Calcium detection dyes?
calcium indicatorcalciumcalcium fluxCa2+GPCRcalbryteCalcium dyes
Can I fix the cells before or after addition of Calcium detection dyes?
calcium indicatorcalciumcalcium fluxCa2+GPCRcalbryteCalcium dyes
Posted on May 20, 2019
Which fluorescent calcium indicators are compatible with GFP positive cells?
Ca2+calcium indicatorcalcium fluxcell assayGPCRCalbrytefluorescent calcium indicatorcalcium mobility
Which fluorescent calcium indicators are compatible with GFP positive cells?
Ca2+calcium indicatorcalcium fluxcell assayGPCRCalbrytefluorescent calcium indicatorcalcium mobility
Posted on April 1, 2019
What is the molar extinction coefficient of RNA?
calcium indicatorcalciumcalcium fluxCa2+GPCRcalbryteCalcium dyesmolar extinction coefficientRNA
What is the molar extinction coefficient of RNA?
calcium indicatorcalciumcalcium fluxCa2+GPCRcalbryteCalcium dyesmolar extinction coefficientRNA
Posted on April 1, 2019
How do you measure Ca2+ response with calcium indicators like Calbryte 520, potassium salt?
calciumcalcium indicator
How do you measure Ca2+ response with calcium indicators like Calbryte 520, potassium salt?
calciumcalcium indicator
Posted on April 1, 2019
How long after addition of agonist can the wells be read by using the Cell Meter™ No Wash and Probenecid-Free Endpoint Calcium Assay Kit (Cat# 36312)?
calciumCa2+calcium indicatorcalcium fluxcalcium mobilitycell assayfluorescent plate readerendpointno washno probenecidGPCRprobenecidorganic anion transporterfluorescent calcium indicator
How long after addition of agonist can the wells be read by using the Cell Meter™ No Wash and Probenecid-Free Endpoint Calcium Assay Kit (Cat# 36312)?
calciumCa2+calcium indicatorcalcium fluxcalcium mobilitycell assayfluorescent plate readerendpointno washno probenecidGPCRprobenecidorganic anion transporterfluorescent calcium indicator
Posted on April 1, 2019
What is the concentration of calcium inside cells?
calciumCa2+calcium indicatorcell assaycalcium fluxcalcium mobilityfluorescent calcium indicator
What is the concentration of calcium inside cells?
calciumCa2+calcium indicatorcell assaycalcium fluxcalcium mobilityfluorescent calcium indicator
Posted on September 26, 2018
What are the three common chemical forms of calcium indicators? What is the difference between salts, dextran conjugates, and acetoxymethyl (AM) esters?
Ca2+calciumcalcium indicatorcalcium mobilitycalcium fluxGPCRcalbrytecalbryte 520fluo-4
What are the three common chemical forms of calcium indicators? What is the difference between salts, dextran conjugates, and acetoxymethyl (AM) esters?
Ca2+calciumcalcium indicatorcalcium mobilitycalcium fluxGPCRcalbrytecalbryte 520fluo-4
Posted on July 9, 2018
Are there any secondary messengers involved in the lysosomal calcium signaling pathway?
Ca2+NAADPcalciumlysosomecalcium fluxcalcium mobilitycalcium indicatorlysosomal calciumsecond messengercalcium concentrationfluorescent calcium indicator
Are there any secondary messengers involved in the lysosomal calcium signaling pathway?
Ca2+NAADPcalciumlysosomecalcium fluxcalcium mobilitycalcium indicatorlysosomal calciumsecond messengercalcium concentrationfluorescent calcium indicator
Posted on July 9, 2018
Are there any secondary messengers involved in the lysosomal calcium signaling pathways?
Ca2+NAADPcalciumlysosomecalcium fluxcalcium mobilitycalcium indicatorlysosomal calciumsecond messengercalcium concentrationfluorescent calcium indicator
Are there any secondary messengers involved in the lysosomal calcium signaling pathways?
Ca2+NAADPcalciumlysosomecalcium fluxcalcium mobilitycalcium indicatorlysosomal calciumsecond messengercalcium concentrationfluorescent calcium indicator
Posted on April 9, 2018
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
GPCRcalciumcalcium fluxcalcium mobilitycalcium indicatorEndoplasmic Reticulum (ER)
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
GPCRcalciumcalcium fluxcalcium mobilitycalcium indicatorEndoplasmic Reticulum (ER)
Posted on February 24, 2018
What is HHBS?
Ca2+calciumcell assaycalcium fluxcalcium indicatormembrane potential
What is HHBS?
Ca2+calciumcell assaycalcium fluxcalcium indicatormembrane potential
Posted on February 1, 2018
What is the molecular weight (mw) of Fluo-4?
fluo-4molecular weightchemicalcalcium indicator
What is the molecular weight (mw) of Fluo-4?
fluo-4molecular weightchemicalcalcium indicator